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Sikdar, Samir Ranjan
- Isolation of Mesophyll Protoplast from Indian Mulberry (Morus alba L) CV. S1635
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1 Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata-700 054, West Bengal, IN
1 Division of Plant Biology, Bose Institute, P-1/12, C.I.T., Scheme VII M, Kolkata-700 054, West Bengal, IN
Source
Journal of Environment and Sociobiology, Vol 13, No 2 (2016), Pagination: 217-222Abstract
Mulberry (Morus alba L. cv S1635) is a perennial crop plant in Indian sericulture and used for silkworm Bombyx mori L. feeding. Qualitative and quantitative improvement of this crop is highly essential in respect to better and quality silk production and hence through breeding approaches the development of new lines is important. Protoplasts are the good source of any plant to study about their genetics and cellular mechanisms for breeding. Using cell wall degradating enzymes mulberry protoplasts from mesophyll tissue were isolated. Several combinations of cellulose, macerozyme and driselase enzymes mixture were tested in different incubation conditions. A combination of Cellulase R10-1%, Macerozyme R10-1% and Driselase-0.25% in 0.6M mannitol showed comparatively better yield (3.8 × 106 / gm tissue) after 3-4 hrs shaking with 100 rpm at 28°C.Keywords
Mulberry Plant, Mesophyll Protoplast, Cell Wall Degradating Enzymes.References
- Asai, T., Stone, J. M., Heard, J. E., Kovtun, Y., Yorgey, P., Sheen, J. and Ausubel, F. M. 2000. Fumonisin B1-induced cell death in Arabidopsis protoplasts requires jasmonate-, ethylene-, and salicylate-dependent signalling pathways. Plant Cell, 12: 1823-1835.
- Bethke, P. C. and Jones, R. L. 2001. Cell death of barley aleurone protoplasts is mediated by reactive oxygen species. Plant J., 25: 19-29.
- Castro, A. 1989. Producción de leche de cabrasalimentadas con King grass (Pennisetum purpureum x P. typloides), suplementadas con diferentesniveles de follaje de Poró (E. poeppigrama) y de fruto de plátano (Musa sp. var. pelipita). Ph.D. thesis, University of Costa Rica, CATIE, 58 pp.
- Cocking, E. C. 1960. A method for the isolation of plant protoplasts and vacuoles. Nature, 187: 927-929.
- Cocking, E. C. 2000.Turning point article plant protoplasts, in vitro cell. Dev. Biol. Plant, 36: 77-82.
- Davey, M. R., Anthoney, P., Brian Power, J. and Love, K. C. 2005, Plant protoplants: status and biochemical perspectives. Biotechnology Advances, 23: 131–171.
- Katagiri, K. 1988. Differences in cell division in mulberry mesophyll protoplast culture due to species and culture conditions. J. Seri. Sci. Japan, 57: 445–446.
- Katagiri, K. 1989. Colony formation in culture of mulberry mesophyll protoplasts. J. Seric. Sci. Japan, 58: 267–268.
- Kim, B. K., Kang, J. H., Jin, M., Kim, H. W., Shim, M. J. and Choi, E. C. 2000. Mycelial protoplast isolation and regeneration of Lentinus lepideus. Life Sci., 66: 1359-1367.
- Koster, K. L., Reisdorph, N. and Ramsay, J. L. 2003. Changing desiccation tolerance of pea embryo protoplasts during germination. J. Exp. Bot., 54: 1607-1614.
- Koyuncu, F. 2004. Organic acid composition of black mulberry. Chemistry of Natural Compounds, 40: 368-369.
- Mandal, P. and Sikdar, S. R. 2003. Plant regeneration from mesophyll protoplasts of Rorippa indica (L.) Hiern, a wild crucifer. Current Science, 85: 1451-1454.
- Ohnishi, T. and Kiyama, S. 1987. Increase in yield of mulberry protoplasts by treatment with chemical substances. J. Seric. Sci. Japan, 56: 407–410.
- Ohyama, K. and Oko, S. 1975. Isolation of protoplasts in Moraceae. Proc. Crop. Sci. Soc. Japan, 44: 121–122.
- Sheen, J. 1999. C4 gene expression. Ann. Rev. Plant Physiol. Plant Mol. Biol., 50: 187-217.
- Sheen, J. 2001. Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol., 127: 1466-1475.
- Tena, G., Asai, T., Chiu, W. L. and Sheen, J. 2001. Plant MAP kinase signaling cascades. Curr. Opin. Plant Biol., 4: 392-400.
- Tewary, P. K. and Lakshmisita, G. 1992. Protoplast isolation, purification and culture in mulberry (Morus spp.). Sericologia, 32: 651–657.
- Tewary, P. K., MohanRam, H. Y. and Rangaswamy, N. S. 1995. Isolation and culture of mulberry (Morus alba L.) protoplasts from callus tissues. Ind. J. Plant Physiol., 38: 114–117.
- Tohjima, F., Yamanouchi, H., Koyama, A. and Machii, H. 1996. Effects of various enzymes on the efficiency of protoplast isolation from mulberry mesophylls. J. Seric. Sci. Japan, 65: 485–489.
- Umate, P., Rao, K. V., Krianmayee, K., Jaya Sree, T. and Sadanandam, A. 2005. Plant regeneration of mulberry (Morus indica) from mesophyll-derived protoplasts. Plant Cell Tissue Organ Cult., 82(3): 289–293.
- Wei, Z., Xu, Z., Huang, J., Xu, N. and Huang, M. 1994. Plants regenerated from mesophyll protoplasts of white mulberry. Cell Research, 4: 183–189.
- Yanagisawa, S., Yoo, S. D. and Sheen, J. 2003. Differential regulation of EIN3 stability by glucose and ethylene signalling in plants. Nature, 425: 521–525.
- Widholm, J. M. 1972. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Tech., 47: 189–194.
- Analysis of Relative DNA Content of Termitomyces Protoplast using Propidium Iodide
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Authors
Affiliations
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, IN
2 Division of Plant Biology, Bose Institute, P-1/12, CIT Rd, Scheme VIIM, Kankurgachi, Kolkata-700054, IN
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, IN
2 Division of Plant Biology, Bose Institute, P-1/12, CIT Rd, Scheme VIIM, Kankurgachi, Kolkata-700054, IN
Source
Journal of Environment and Sociobiology, Vol 17, No 2 (2020), Pagination: 15-18Abstract
Towards establishment of biodiversity character via analysis of relative DNA content of wild edible mushroom, Termitomyces species is used here for the first time. Dye propidium iodide (PI), an intercalating fluorescent molecule with molecular mass of 668.4 Da is used for staining of Termitomyces protoplasts to analyse the cellular DNA content using flow cytometer. PI binds the cellular nucleic acids at 535 nm excitation and 617 nm emission range. The protoplasts were isolated from liquid MYG grown vegetative tissues of edible mushroom strain Termitomyces heimii. The isolated protoplasts were purified with 0.6M manitol (MW 3350) followed by washing in PBS buffer. Protoplast pellet was suspended in PI (l mg/ml) hypotonic solution and final volume made up with sterile water. Incubation was done at 37°C for 30 min followed by analyzing using flow cytometer. Results showed that staining depends upon the protoplast purity, size and viability. The vegetative tissue showed a heterogeneous protoplast population in terms of sizes which is ultimately reflected on the relative amount of DNA content. The heterogeneous protoplast population in the suspension through cytometry score results the unequal amount of DNA content in every cell meaning unequal size, age and viability. It is established that PI can be used for staining of mushroom protoplast in biodiversity and genetic research.Keywords
Edible mushroom, Protoplast, Propidium iodide, Flow cytometry, DNA content.- Effect of Biochemicals from different Cultivars of Indian Mustard (Brassica Juncea L.) on the Population Abundance of Lipaphis Erysimi (Kalt.) in Laboratory Condition
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Authors
Affiliations
1 School of Biotechnology and Biosciences, Department of biotechnology, 398, Ramkrishnapur Road, Brainware University, Barasat, West Bengal: 700 125, IN
2 Bose Institute, Division of Plant Biology, P-1/12, C. I. T. Road, Scheme VII M, Kolkata-700 054, West Bengal, IN
1 School of Biotechnology and Biosciences, Department of biotechnology, 398, Ramkrishnapur Road, Brainware University, Barasat, West Bengal: 700 125, IN
2 Bose Institute, Division of Plant Biology, P-1/12, C. I. T. Road, Scheme VII M, Kolkata-700 054, West Bengal, IN
Source
Journal of Environment and Sociobiology, Vol 17, No 2 (2020), Pagination: 31-36Abstract
Population abundance of mustard aphid Lipaphis erysimi (Kalt.) was studied upon the treatment of aqueous extract from eight cultivars of Indian mustard Brassica juncea L. from different agronomical regions of India. A wild crucifer Rorippa indica, known for its aphid tolerant nature, also was studied, side by side. Treatment of R. indica extract on the isolated population of mustard aphids showed a significant suppressing effect on aphid population. Cultivar Gujarat Mustard 3 was appeared to be best host for the aphid, and cv. Ashirwad was seen to be most tolerant to L. erysimi as on the treatment of water extract from Gujarat Mustard 3.Nymphal growth of aphid was not affected and a regular growth pattern was observed here. But aqueous extract treatment from cv. Ashirwad significantly controlled aphid population on non-host Nicotiana tabacam.Keywords
Mustard Aphids, Brassica Juncea, Lipaphis Erysimi, Rorippa Indica, Aqueous Extract.- Analysis of Genetic Diversity of Protoplast Fused Mushrooms Pleurotus Florida X Lentinus Squorrosulus (PFLS) Somatic Hybrids through Rapd Markers
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Authors
Affiliations
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, IN
2 Division of Plant Biology, Bose Institute, P-1/12, CIT Rd, Scheme VIIM, Kankurgachi, Kolkata-700054, IN
1 Department of Biotechnology, School of Biotechnology & Biosciences, Brainware University, 398, Ramkrishnapur Road, Barasat, North 24 Parganas, Kolkata-700125, IN
2 Division of Plant Biology, Bose Institute, P-1/12, CIT Rd, Scheme VIIM, Kankurgachi, Kolkata-700054, IN
Source
Journal of Environment and Sociobiology, Vol 17, No 2 (2020), Pagination: 37-43Abstract
A total of twelve somatic hybrid strains, Pleurotus florida x Lentinus squorrosulus (pfls), were characterized through PCR based RAPD analysis. Sixteen random decemer primers yielded a total of 136 fragments with 97.79 % polymorphism with an amplicon range of 200 bp - 3000 bp in size. RAPD-08 showed optimum amplification and generated 12 scorable bands (250 bp -1800 bp) where RAPD-07 amplified least and generated 5 scorable bands (400 bp - 1300 bp). PIC ranged from 0.424 (SRS-15) to 0.499 (SRS-16). Based on Jaccard’s proximity matrix the highest genetic distance was found in between parent L. squarrosulus and hybrid pfls1q which means they are genetically most distantly related. Among the hybrid pfls1k and pfls1p showed maximum genetic distance while the most closely related hybrids were pfls1i and pfls1l. The parent L. squarrosulus was most genetically distant individual than the other parent P. florida.Keywords
Hybrid Mushroom, Genetic Diversity, Polymorphism, RAPD.- Fatty Acid Profile Analysis of Abci Somatic Hybrid Mushroom Strains using GC-MS Technique
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Authors
Affiliations
1 Division of Plant Biology, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata-700 054, IN
1 Division of Plant Biology, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata-700 054, IN